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OF OPTICAL MICROSCOPY IN CELL BIOLOGY |
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I V S y m p o s i u m
27-30 April 1998
Università degli Studi di Pavia
Dipartimento di Biologia Animale
Piazza Botta, 9-10
Coordinators
Prof. Isabel Freitas and Prof. Carlo Pellicciari,
University of Pavia
Dr. Heinz Gundlach, Carl Zeiss, Oberkochen
Il Simposio
ha lo scopo di illustrare come gli sviluppi delle tecniche di Microscopia Ottica
siano strettamente collegati e contribuiscano al miglioramento delle conoscenze
nel campo della Biologia Cellulare. Esso si presenta inoltre come un forum
interdisciplinare atto ad accogliere critiche e suggerimenti sugli obiettivi futuri
delle due branche della Scienza. Nel corso del Simposio si svolgeranno dimostrazioni
pratiche del funzionamento di un microscopio confocale e di sistemi di acquisizione,
elaborazione ed analisi di immagini.
The Symposium
has the purpose of illustrating how the developments of optical microscopy techniques
are closely related and contribute to the widening of knowledge in Cell Biology.
It presents itself furthermore as an interdisciplinary forum to gather criticisms
and suggestions on the future goals of the two branches of Science. During the
Symposium practical demonstrations will be made of the functioning of a confocal
microscope and of systems of image acquisition, processing and analysis.
PROGRAM
The seminars will be held on the classroom of the
1st floor of the Institute of Zoology
The workshops will be made on the classrooms on
the ground floor of the Institute of Zoology
Piazza Botta, 9 27100 Pavia
27th April: Digital fluorescence microscopy and laser scanning microscopy
9:30 Opening of the Symposium (G. Gerzeli, I. Freitas, Univ.
Pavia; H. Gundlach, Carl Zeiss, Oberkochen)
9:45 Possibilities and limits of fluorescence microscopy.
Digital fluorescence microscopy and laser scanning microscopy (H. Gundlach;
Carl Zeiss, Oberkochen)
10:45 Introduction to laser scanning microscopy (E. Negri; Carl
Zeiss, Milano)
11:30 Image analysis in two and three dimensions (E. Negri; Carl
Zeiss, Milano)
14:30 Workshop: Application of confocal microscopy to specific problems
(E. Negri; Carl
Zeiss, Milano)
28th April: Apoptosis
9:30 Nuclear and cytoplasmic events during apoptosis (S. Barni
et al.; Univ. Pavia)
10:15 The apoptotic cells: from membrane to nucleus (E. Falcieri
et al.; Univ. Urbino)
11:15 Fate of nuclear ribonucleoproteins during apoptosis (C. Pellicciari
et al.; Univ. Pavia)
11:30 In situ detection of Reactive Oxygen Species in apoptotic
cells (I. Freitas et al.; Univ. Pavia)
11:45 Histochemical tracking of apoptotic cells after engulfment
by scavenger or antigen presenting cells (G. Galati et al.; S. Raffaele
Hosp., Milano)
14:30 Workshop: Application of confocal microscopy and image analysis
to the study of apoptosis (E. Negri; Carl Zeiss, Milano)
29th April: New techniques in fluorescence microscopy
9:30 "Green Fluorescent Protein" (GFP) and its spectral mutants
in the study of cell function (R. Rizzuto et al.; Univ. Padova)
10:15 Two-multi-photon excitation in 3D laser scanning fluorescence
microscopy (A. Diaspro; INFM, Univ. Genova)
11:20 Time-resolved fluorescence microscopy (G. Bottiroli; CNR Center
for Histochemistry, Pavia)
12:00 General discussion
14:30 Workshop: "Basic Elements of Image Analysis and Treatment of
Digital Images" (V. Bertone, Univ. Pavia)
30th April: New fontiers of Fluorescence In Situ Hybridization (FISH)
9:30 Use of minichromosomes (MCs) in gene therapy (E. Raimondi;
Univ. Pavia)
10:00 Resources for molecular cytogenetics and their applications
(M. Rocchi et al.; Univ. Bari)
10:45 FISH procedures in chromosome structure research (S.
Garagna et al.; Univ. Pavia)
11:40 Application of in situ hybridization to the study
of insect polytene and mitotic chromosomes (C. Torti et al.; Univ. Pavia)
14:30 Multi Color FISH Analysis - from 24 Color Karyotyping to High
Resolution Color Banding (A. Plesch; MetaSystems, Altlussheim)
15:15 Workshop: Demonstration of various CCD cameras and correspondent
software (A. Plesch; MetaSystems, Altlussheim)
In collaboration with:
Research Doctorate in Cell and Animal Biology
Research Doctorate in Genetic Sciences (Genetics and Molecular Biology)
School of Specialization in Applied Genetics
School of Specialization in Hematology
School of Specialization in Clinical Pathology
Polaroid (Italia) SpA
Under the auspices of the Italian Society of Histochemistry
This page was made by Vittorio Bertonebertone@unipv.it