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AND PHOTOMICROGRAPHY TECHNIQUES |
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V
B a s i c Co u r s e
20-24 April 1998
Università degli Studi di Pavia
Dipartimento di Biologia Animale
(Direttore: Prof. G. Gerzeli)
Coordinators
Dr. Heinz Gundlach, Carl Zeiss, Oberkochen
Prof. Isabel Freitas and Prof. Carlo Pellicciari,University
of Pavia
Il Corso,
rivolto principalmente a Dottorandi, Specializzandi e Ricercatori operanti in
campo biomedico, si prefigge di fornire le basi delle tecniche di microscopia
ottica, anche in fluorescenza, delle modalità di miglioramento del contrasto
ottico e della fotografia al microscopio. Le nozioni teoriche illustrate
nelle lezioni del mattino verranno messe in pratica, per gruppi di lavoro,
nelle sessioni “workshop” del pomeriggio.
The Course,
addressed mainly to PhD and Specialization students and Researchers in the biomedical
field, aims at providing the basis of optical microscopy techniques, including
fluorescence, of the modalities for the enhancement of the optical contrast,
and of photomicroscopy. The theoretical notions given in the morning lessons
will be applied, by groups of partecipants, during the afternoon workshops.
PROGRAM
The seminars will be held on the classroom of the
1st floor of the Institute of Zoology
The workshops will be made on the classrooms on
the ground floor of the Institute of Zoology
Piazza Botta, 9 27100 Pavia
Monday, 20th April
9:30 Basic concepts of geometric Optics - light paths in the
microscope, magnification
10:30 Illumination according to Prof. August Köhler
11:15 Design of a light microscope
14:30 Workshop: Application of the morning topics
Tuesday, 21st of April
9:30 Theory of image formation in the light microscope according
to the experiments of Ernst Abbe
11:00 Illumination methods - bright field, dark field, oblique illumination,
phase contrast, differential interference contrast (DIC), polarization
Workshops
14:30 Experiments on the theory of image formation according
to Ernst Abbe
15:15 Techniques for contrast enhancement
Wednesday 22nd of April
9:30 Basics in photomicrography: principle, instrumentation,
color and black&white films, filters
11:00 Video microscopy for digital imaging and documentation
Workshops
14:30 Photomicrography
15:30 Digital imaging including all optical contrast enhancement
techniques
Thursday 23rd of April
9:30 Fluorescence microscopy - principle, instrumentation
and applications
10:30 Conventional multiple fluorescence - possibilities and limits
11:30 Digital fluorescence imaging - principle and applications
Workshops
14:30 Setting up the fluorescence microscope
15:30 Photomicrography in fluorescence microscopy as well as in
all other contrast enhancement techniques
Friday 24th of April
9:30 Introduction to laser scanning microscopy (LSM), confocal imaging and image analysis
Workshop
11:00 Demonstration of the LSM in groups
Free practical work
14:30 Final discussion and evaluation of the photomicrographs taken
by the partecipants
In collaboration with:
Research Doctorate in Cell and Animal Biology
Research Doctorate in Genetic Sciences (Genetics and Molecular Biology)
School of Specialization in Applied Genetics
School of Specialization in Hematology
School of Specialization in Clinical Pathology
Polaroid (Italia) SpA
Under the auspices of the Italian Society of Histochemistry
This page was made by Vittorio Bertone bertone@.unipv.it