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VII Basic Course
Light microscopy and photomicrography techniques
Pavia, May 2-5, 2000
Dipartimento di Biologia Animale
University of Pavia - Piazza Botta 9/10
Organizers
Isabel Freitas & Carlo Pellicciari, University
of Pavia, Italy
Heinz Gundlach, Carl Zeiss Oberkochen, Germany
P R O G R A M
The lectures will be given in the Aula Spallanzani at the 1st floor
and the afternoon workshops in the laboratory at the ground floor
Laboratory of Zoology, Piazza Botta 9
Tuesday, May 2th 2000
Morning
9:30 Introduction to light microscopy: geometric light path,
magnification, 1 step imaging, construction of a light microscope.
10:30 Illumination according to Prof. August Köhler.
11:15 Design of a light microscope: stand, lenses, eyepiece, condenser,
light source
Afternoon
14:30 Workshop: Application of the morning topics
Wednesday, May 3rd, 2000
Morning
9:30 Theory of image formation in the light microscope according
to the experiments of Ernst Abbe.
11:00 Illumination methods: bright field, dark field, oblique illumination,
phase contrast, differential interference contrast (DIC), polarization.
Afternoon
14:30 Experiments on the theory of image formation (Video microscopy
session).
Workshop: Optical contrast enhancement techniques
Thursday, May 4th 2000
Morning
9:30 Basics in photomicrography: principle, instrumentation,
color- and black-and-white films, filters.
11:00 Video microscopy for electronic documentation and digital
imaging
Afternoon
14:30 Workshop: Photomicrography and digital imaging, including
all optical contrast enhancement techniques
Friday, May 5th, 2000
Morning
9:30 Fluorescence microscopy: principle, instrumentation and
applications
11:00 Digital fluorescence imaging: principle, instrumentation and
applications
Afternoon
14:30 Workshop: Fluorescence microscopy. Photomicrography in fluorescence
microscopy.
16:30 Final discussion and presentation of the results obtained
by the partecipants
In collaboration with:
Dottorato di Ricerca in Biologia Cellulare e Animale
Scuola di Specializzazione in Genetica Applicata
Under the auspices of the Società Italiana di
Istochimica
and the Società Italiana di Microscopia Elettronica
clicking -------------------------->> HERE bertone@unipv.it |
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Symposium page
This site is created and maintained by Vittorio
Bertone
Last version 19 April 2000