Continuing education course
LIGHT MICROSCOPY
AND
PHOTOMICROGRAPHY TECHNIQUES

VI
B a s i c   Co u r s e

26-30 April, 1999
 

 Università degli Studi di Pavia
Dipartimento di Biologia Animale
(Direttore: Prof. G. Gerzeli)

Coordinators
 Dr. Heinz Gundlach, Carl Zeiss, Oberkochen
Prof. Isabel Freitas and Prof. Carlo Pellicciari,
University of Pavia


  Il Corso, rivolto principalmente a Dottorandi, Specializzandi e Ricercatori operanti in campo biomedico, si prefigge di fornire le basi delle tecniche di microscopia ottica, anche in fluorescenza, delle modalità di miglioramento del contrasto ottico e della fotografia al microscopio.  Le nozioni teoriche illustrate nelle lezioni del mattino verranno messe in pratica, per gruppi di lavoro,  nelle sessioni “workshop” del pomeriggio.

  The Course, addressed mainly to PhD and Specialization students and Researchers in the biomedical field, aims at providing the basis of optical microscopy techniques, including fluorescence, of the modalities for the enhancement of the optical contrast, and of photomicroscopy. The theoretical notions given in the morning lessons will be applied, by groups of partecipants, during the afternoon workshops.


   

PROGRAM

The Course will be held at the Laboratory of Zoology

The seminars will be given in the Aula Spallanzani at the 1st floor
and the workshops in the laboratory at the ground floor
Piazza Botta, 9   27100  Pavia




 Monday, April 26th

  9:30 Basic concepts of geometric Optics - light paths in the microscope, magnification
10:30 Illumination according to Prof. August Köhler
11:15 Design of a light microscope

Workshop
14:30 Application of the morning topics

 Tuesday, April 27th

  9:30 Theory of image formation in the light microscope according to the experiments of Ernst Abbe
11:00 Illumination methods - bright field, dark field, oblique illumination, phase contrast, differential interference contrast (DIC), polarization

Workshops
14:30 Experiments on the theory of image  formation according to Ernst Abbe
15:15 Techniques for contrast enhancement

Wednesday, April 28th

  9:30 Basics in photomicrography: principle, instrumentation, color and black&white films, filters
11:00 Video microscopy for digital imaging and documentation
 

Workshops
14:30 Photomicrography
15:30 Digital imaging including all optical contrast enhancement techniques

Thursday, April 29th

  9:30 Basics in Fluorescence (G. Bottiroli, CNR, Pavia)
10:30 Conventional fluorescence microscopy (G. Bottiroli, CNR, Pavia)
11:30 Digital fluorescence imaging - principle and applications

Workshops
14:30 Setting up the fluorescence microscope
15:30 Photomicrography in fluorescence microscopy as well as in all other contrast enhancement techniques

Friday, April 30th

  9:30 Introduction to laser scanning microscopy (LSM), confocal imaging and image analysis

Workshop
11:00 Free practical work
 

14:30 Final discussion and evaluation of the photomicrographs taken by the partecipants
 
 




 

In collaboration with:
Research Doctorate in Cell and Animal Biology
 

Under the auspices of the Italian Society of Histochemistry

Se sei interessato a partecipare al Corso mandaci una e-mail
specificando
- il tuo nome
- la tua Istituzione di appartenenza

If you are interested in attending the Course, please send us an
e-mail message with
- your name
- your Institution

clicking --------------------------------------- HERE   anatcomp@unipv.it
 

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This site is created and maintained by Vittorio Bertone
Last version 12 April 1999