Continuing Education Course

MICROSCOPY TECHNIQUES
FOR STUDYING APOPTOSIS AND OXIDATIVE STRESS

December 2-3, 1999

University of Pavia
Department of Animal Biology
 
 


 
 

Scientific results
Rat liver (cryosection). Autofluorescence, excitation with UV light. False colors. Red: vitamin A (original fluorescence: blue-green). Green: other autofluorescent compounds such as NAD(P)H in hepatocytes and collagen in connective tissue (original fluorescence: green) Isolated hepatocytes: double staining with FITC-labeled anti-actin and DAPI
Human Hep-2 cells: double staining with FITC-labeled anti-Bromodeoxyuridine and DAPI Apoptotic Hep-2 cells after actinomycin-D treatment: double staining with sulphorhodamin and Hoechst 33258

 
Hepatocytes stained with Alexa 488 Annexin V and propidium iodide to detect apoptotic cells (green fluorescence) and dead cells (red and green fluorescence) Frozen liver section stained with Alexa 488 Phalloidin (green fluorescence) for labeling F-actin and Hoechst 33342 (blue fluorescence) for labeling DNA

 
Images were taken with a Hamamatsu ORCA cooled CCD camera on a Zeiss Axioplan II microscope using 20X, 40X and 63X oil immersion PlanApo objectives

P a r t e c i p a n t s

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Last version 07 March 2000