X  Basic Course on Light Microscopy and Photomicrography Techniques

Monday, 16 June
  9:30 Basic concepts of geometric optics - light paths in the microscope, magnification
10:30 Illumination according to Prof. August Köhler
coffee break
11:15 Design of a light microscope: stand, lenses, eyepiece, condenser, light source

14:00 Workshop: Application of the morning topics: Köhler illumination practice
15:00 Theory of image formation in the light microscope according to the experiments of Ernst Abbe

 Tuesday, 17 June
  9:30 Illumination methods - bright field, dark field, oblique illumination, phase contrast, differential interference contrast (DIC)
coffee break
11:00 Workshop: contrast enhancement

14:15 Basics in photomicrography: principle, instrumentation, color and black&white films, filters, digital cameras
15:30 Workshop: applications in photomicrography
 

Wednesday, 18 June
  9:30 Basics in Fluorescence (G. Bottiroli)
10:45 Supravital Fluorochromes (S. Barni)
coffee break
11.15 Introduction to Digital Imaging (V. Bertone)

14:30 Workshops Photomicrography (traditional and digital)
16:00 Introduction to laser scanning microscopy (LSM), confocal imaging and image analysis (P. Vaghi)

Thursday, 19 June
 9:30 Fluorescence microscopy: instrumentation and applications
10:30 Multicolor fluorescence detection
coffee break
12.00 Digital detection of low fluorescence signals: application to FISH

14:30 Inverted microscopy
15:30 Workshops Setting up the fluorescence microscope
15:30 Photomicrography in fluorescence microscopy as well as in all other contrast enhancement techniques

Friday, 20 June
  9:30 LSM, confocal imaging practice
 

Le lezioni, quando non diversamente specificato, sono tenute in inglese dal Dr. Heinz Gundlach. All'occorrenza un servizio di traduzione estemporaneo verrà attivato per meglio precisare i concetti più importanti.

PICTURES OF THE

PARTICIPANTS

BACK to the ANATOMIA COMPARATA E CITOLOGIA site

This site is created and maintained by Vittorio Bertone
Last version 26 MAG 2003